The viability of acute brain slices is typically confined to a 6–12 hour window, which constrains the duration of experiments. Our team has developed an innovative recovery incubation technology that successfully prolongs this period to over 36 hours. The mechanism involves temperature regulation of the incubated artificial cerebral spinal fluid (aCSF), coupled with its continuous circulation through a UVC filtration system. Concurrently, it monitors both temperature and pH levels. This dual approach of temperature management and UVC filtration effectively suppresses bacterial growth, keeping it in the initial lag phase, and as a result, significantly enhances the longevity of brain slices. The effectiveness of the brain slices was confirmed using electrophysiological measurements and assessments of cell viability. By offering consistent monitoring of incubation parameters and creating a standardized setting, this innovation aids researchers in maintaining usable tissue samples for extended experimental work, effectively cutting down on the preparation time for brain slices and reducing the number of animals needed for studies.